Cloned (Comment) | Organism |
---|---|
expression of isozyme AKR1C21 in Escherichia coli strain JM109 | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant isozyme AKR1C21, hanging drop vapour diffusion method, 16 mg/ml enzyme in 10 mM Tris-HCl, pH 7.5, and 2 mM 2-mercaptoethanol, mixed with an equal volume of 0.003 ml of reservoir solution containing 0.1 M HEPES, pH 7.5, 10% PEG 6000, 5% 2-methyl-2,4-pentanediol, equilibration against 1 ml reservoir solution, 22°C, 1 week, X-ray diffraction structure determination and analysis at 1.8 A resolution, molecular replacement | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, recombinant isozyme AKR1C21, SDS-PAGE | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
isozyme AKR1C21 | - |
Purification (Comment) | Organism |
---|---|
recombinant isozyme AKR1C21 from Escherichia coli strain JM109 to homogeneity | Mus musculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, recombinant isozyme AKR1C21, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
3(17)alpha-hydroxysteroid dehydrogenase | - |
Mus musculus |
AKR1C21 | - |
Mus musculus |